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Thermo Fisher
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MACHEREY NAGEL
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Image Search Results
Journal: Chemistry & Biology
Article Title: The Iterative Gramicidin S Thioesterase Catalyzes Peptide Ligation and Cyclization
doi: 10.1016/j.chembiol.2006.10.011
Figure Lengend Snippet: Dimerization and Cyclization of Pentapeptidyl- and Decapeptidyl-Thioesters Mediated by the GrsB PCP-TE (A) Incubation of GS10 (300 μM) with PCP-TE (10 μM) for 60 min. (B) Incubation of GS5 SNAC (300 μM) with PCP-TE (10 μM) for 60 min. (C) Incubation of GS5 SPh (300 μM) with PCP-TE (10 μM) for 60 min. The blue HPLC trace corresponds to the assay with enzyme, and the red trace shows incubation of the substrate in the absence of the enzyme. Analysis was performed on a C 18 Nucleodur column (Macherey and Nagel; 250/2; pore diameter, 100 Å; particle size, 3 μM) with the following gradient: 0–40 min, 5%–60% MeCN/0.1% TFA into H 2 O/0.1% TFA, 40–45 min, 60%–95% MeCN/0.1% TFA into H 2 O/0.1% TFA, 0.3 ml/min, 45°C.
Article Snippet: Analysis was performed on a
Techniques: Incubation